Long-read Nanopore Sequencing-based Draft Genome of a Carbapenem-resistant Pseudomonas aeruginosa.

Abstract Objectives Pseudomonas aeruginosa (P. aeruginosa) is a common Gram-negative bacterium that can cause a series of serious infections, such as lower respiratory tract infection, urinary-tract infections with catheterized patients. Here we reported the draft genome of carbapenem-resistant P. aeruginosa. Methods We described the genome of the P. aeruginosa using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. Results The resulting assembly was highly contiguous, containing a total of 6,624,003 bp with a GC content of 66.21%. Annotation identified 6,389 protein-coding genes. Mutations in oprD and MexR gene conferred resistance to carbapenems to the P. aeruginosa isolate. Conclusions The draft genome sequence of carbapenem-resistant P. aeruginosa could provide a solid basis for further research on the resistance mechanism and the development of drug therapy for drug resistant genes.