Programmable RNA methylation and demethylation using PUF RNA binding proteins

New methods to control local RNA methylation are needed to elucidate the function of individual m6A. Here, fusion proteins between the programmable RNA binding protein PUF and the m6A demethylase FTO or METTL14 methyltransferase were designed. FTO-PUFs and METTL14-PUFs showed sequence-specific RNA demethylation and methylation activities, respectively.