A rapid method for measuring serum amyloid A protein by latex agglutination nephelometric immunoassay

Serum amyloid A protein (SAA) is a sensitive acute phase react ant. We developed a method for the rapid measurement of human SAA in serum by kinetic nephelometry of anti-SAA antibody-coated latex agglutination. Measurement takes less than 6 min using an automated analyser. Standardization of the assay employs SAA-enriched high density lipoprotein as the primary standard. The values determined by our new method and by conventional enzymeimmunoassay showed good agreement (r = 0·988). The normal range was 0·17–10·0 mg/L [mean (SD)]. This rapid method should prove useful in clinical laboratories.