Development of 3D in vitro co-culture model to increase drug selectivity for humans.

Insulin resistance is a metabolic state where insulin sensitivity is lower than normal condition and strongly related to type 2 diabetes. However, in vitro model mimicking insulin resistance is rare and thus screening drugs for insulin resistance severely depend on in vivo model. Here we present a novel 3D ADMSC and macrophage co-culture model to increases the drug selectivity for humans with in-house fabricated co-culture plate. Our 3D co-culture model with/without 3D co-culture plate can successfully mimic insulin resistance while allowing investigation of anti-obesity or anti-diabetic drugs effects on each co-culturing cell type. This co-culture system can potentially be applied to the development of functional analogs in other tissues. This article is protected by copyright. All rights reserved.