Predigested high-fat meats based on Lactobacillus fermentum lipase enzyme immobilized on silver-alginate nanoparticle matrix

Lipase enzyme plays a part in the environmental and pharmaceutical industries, microbial lipase is the most potential source for industrial application. Lipases were extracted from endogenous isolate, sequentially purified by ammonium sulphate and Sephadex G-150 methods, and immobilized on silver-alginate nanoparticles to test the ability to used preparing predigested of high-fat meat to low fat meat. Lipase producing bacteria was identified as Lactobacillus fermentum, that extracted mesophilic lipases presented maximal activity at pH 8 and 40 °C. The purified enzyme was recovered up to 16% yield in 1.4 fold purification and specific activity 4 U/mg. Lipase activity exhibited the highest inhibition rate by adding 1% of non-ionic detergent of Triton X-100, Tween 80 and Tween 20 (60, 63 and 71%, respectively). Silver-alginate nanoparticles is a potential matrix for immobilization of biomolecules, especially enzymes. Immobilized lipase was preserved; the specific activity (2.3–2 U/mg) can even be reused more than three times, which attributed to hydrophobicity and flexibility of lipases. Predigested meat was obtained from treating high-fat meat with immobilized enzymes, as shown by losses of 40% of initial meat weight after 14 days of lipid digestion. Conjugation of silver-alginate matrix with lipase can controlled the digestion of meat fat and easy to get rid of in comparing to expressed enzyme. Concept of predigested meat could revolutionize the world of high-fat diets without interfering their dietary habits.