Cardioprotective effects of adenosine within the border and remote areas of myocardial infarction

Background: Adenosine may have beneficial effects on left ventricular function after myocardial infarction (MI), but the magnitude of this effect on remote and MI areas is controversial. We assessed the long-term effects of adenosine after MI using electrocardiogram-triggered 18 F-fluorodeoxyglucose positron emission tomography. Methods: Wistar rats were subjected to coronary ligation and randomized into three groups treated daily for 2 months by NaCl (control; n= 7), 2-chloroadenosine (CADO; n= 8) or CADO with 8-sulfophenyltheophilline, an antagonist of adenosine receptors (8-SPT; n= 8). Results: After 2 months, control rats exhibited left ventricular remodelling, with increased end-diastolic volume and decreased ejection fraction. Left ventricular remodelling was not significantly inhibited by CADO. Segmental contractility, as assessed by the change in myocardial thickening after 2 months, was improved in CADO rats compared to control rats (+1.6% ± 0.8% vs. �2.3% ± 0.8%, p < 0.001). This improvement was significant in border (+5.6% ± 0.8% vs. +1.5% ± 0.8%, p< 0.001) and remote (�4.0% ± 1.0% vs. �10.4% ± 1.3%, p< 0.001) segments, but absent in MI segments. Histological analyses revealed that CADO reduced fibrosis, cardiomyocyte hypertrophy and apoptosis. Protective effects of CADO were blunted by 8-SPT. Conclusion: Long-term administration of adenosine protects the left ventricle from contractile dysfunction following MI.