Efficient CRISPR/Cas9-based plant genomic fragment deletions by microhomology-mediated end joining.

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein (Cas) systems produce double strand breaks (DSBs) in targeted sites and generally cause insertion/deletions of one or several bases by non-homologous end joining (NHEJ)-mediated DNA repair. However, many edited cell lines with small insertions/deletions produce abnormal transcripts or proteins causing unexpected effects that complicate functional analysis (Tuladhar et al., 2019).