Infection cycle of maize stalk rot and ear rot caused by Fusarium verticillioides

Fusarium verticillioides, an important maize pathogen produces fumonisins and causes stalk and ear rot; thus, we are aimed to clarify its infection cycle by assessing enhanced green fluorescent protein (EGFP) expression in stalk and ear rot strains. Maize seeds were inoculated with stable and strongly pathogenic transformants. To investigate the degree of infection, inoculated plants were observed under a stereo fluorescence microscope, and affected tissue strains were detected using PCR. We found that both transformants infected maize. Hyphae infected the plants from radical to the stem and extended to the ear and infected ear kernels caused a second infection. This process formed the infection cycle.