The Breast Milkâs Hsa-miR-195-5p is a WÅ½ÆÄnÆÄÅ¯ Biomarker for the WrÅ½ÆÄcÆÅ½nagainst Mother-to-Child Transmission of HIV-1

Linda Mouafo Mekue,Céline Nguefeu Nkenfou,Lorena Zentilin,Jules-Roger Kuiate,Mauro Giacca,Alexis Ndjolo

The Breast Milkâs Hsa-miR-195-5p is a WÅ½ÆÄnÆÄÅ¯ Biomarker for the WrÅ½ÆÄcÆÅ½nagainst Mother-to-Child Transmission of HIV-1

2019

Background: Some factors have been described to impact mother-to-child transmission of HIV. It is known that it is a cA½mbAnAtiA½n of factors that can explain transmission or AErA½AEActiA½nI Other factors such as miRNA can be used as a marker for understanding and AErAAActinA the AEAAE©Arn of many diseases. The aim of this study was to look at host or viral miRNA that can be used as a marker to predict the transmission or AErACAntiA½n of the HIV-1 from mother-tochild. Methods and A®nAAnAAEI The breast milk and plasma samples of 13 AErAnAEmAAE©ArAEI 56 nA½nI²AErAnAEmAAE©ArAE and 15 HIV non-infected mothers were collected. The AAA«ArAntiAA¯ expression levels of seven human miRNAs (HsamiR-29a-3p, Hsa-miR-29b, Hsa-miR-28-3p, HsamiR-125a-5p, Hsa-miR-149-3p, Hsa-miR-195-5p, HsamiR-191-5p) and one viral miRNA (HIV-miR-N367) were analyzed by miRNA rAAA¯I²timA PCR. Breast milk’s HsamiR-195-5p (p=0.009) and Hsa-miR-191-5p (p=0.003) were upregulated in the nA½nI²AErAnAEmAAE©Ar mothers. Plasma Hsa-miR-28-3p (p=0.04) was upregulated in nA½nI² AErAnAEmAAE©Ar mothers as well. The Hsa-miR-195-5p nAAAtiCAA¯y correlate with the CD4+ count lower than 300 (r=-0.91, p=0.005) and was AAAntiA®AA as a miRNA having the AEA½AEAntiAA¯ to AAAEtinAAEµAAEA between AErAnAEmAAE©Ar and nA½nI² AErAnAEmAAE©Ar mothers. Conclusion: This study has shown for the A®rAEAE timA the AmAEA¯AcAtiA½n of breast milk’s Hsa-miR-195-5p in AErA½AEActinA newborns from acquiring HIV AnA¨ActiA½nI This miRNAs will be further evaluated in a larger AEA½AEAEµA¯AtiA½n of AErAnAEmAAE©ArAE and nA½nI²AErAnAEmAAE©ArAE in order to set a cAEµAEA½A« for clinical AxAEA¯A½AAEAtiA½nI ^AAnAA®cAncA of the study: Expression of Hsa-miR-195-5p in the breast milk’s could be used as a biomarker to predict the outcome of Mother-to-child transmission of HIV.

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