A DNA SIZE PROBE BASED ON ENERGY MIGRATION IN CROSSLINKED CHROMATIN

DNA sizing has a central role in virtually every aspect of physical genomic analysis. Although capillary gel electrophoresis adequately separates DNA samples, accurate sizing of short sequences is problematic. Here we introduce a simple methodology that affords sizing of short DNA sequences at 2nt resolution via wrapping and linking histone subunits with fluorophore-tagged ssDNA to extract a wrapping and linking ratio that is unique to the reported DNA size.