Influence of lodoacetate on glycolytic intermediates and on respiration in ehrlich-lettre ascites carcinoma cells

Abstract Glycolytic intermediates have been determined in whole tumor and tumor cells, before and after washing, at intervals of time after glucose and glucose plus iodoacetate (IAA) addition to washed tumor cells. The influences of IAA on glycolysis and respiration have been determined on washed mitochondria plus cell sap. The effects of added α-glycerophosphate (α-GP), pyruvate, and both on glycolytic intermediates and respiration of washed whole cells and of mitochondria plus cell sap also have been determined, and the oxidation of α-GP and appearance of dihydroxyacetone phosphate (DHAP) were measured in five-times washed mitochondria. The findings definitely show that the addition of an optimum amount of iodoacetate to either whole cells or mitochondria plus cell sap causes an increased accumulation of DHAP and α-GP, as well as pyruvate, which in turn is responsible for a stimulation of respiration.