ALTERATIONS IN THE EXPRESSION OF THE PROTEIN-KINASE-C ISOTYPES IN HUMAN CELL-LINES AND TISSUE BIOPSIES

·Protein Kinase C: Regulation, Structurei Function and Role In Human D~ QZ 411 ALTE!\ATlOnS ~=~ Tl!£ EX?R.ESSION CF THE PRCTS:U: KIN.'\S.E C !SC.T'.:'PES IN HUM.a.~ CSJ:!.. L!NES i\NP '!'ISSU!:: 8 .l()PS-H:~. Couqlas T. '.:'ar.:a::i:shi, Shigeo Ohno-, Jan:l!'s Jakowat.z.,. Ma.Ct.hew Gc~: ·J =4:-l, and Franit. l . :~eY~·k~r::! j~., ·c1 fni~al Cance!' Cent:er, U.C. H~vine , I r•,ine 1 CA 92''17, and Cept.. ol Kol. B~o! .. , '!ckoha- City Cn!versir.y l:cnoo.i c t Medic ine, ¥okoharna, i3~, Japan;. previo s studies . on th ' expression of t:ht' p·rot'e in lttr:1ase c (l'!(C) isor.ypes (a, jl, y, ar.d rl Our 1.n b'\1man ·i;irirnary melanocy~es and metast.a ·t ic melenOlllll eel!' s .trains had 'shown t.hat ,.e~astat.ic melanoma. cells did not e!q:i:::ess PKC ~ !' l\!lA; tfanscripts usin9 'Northern blot h.ybrldi~·a·t ior. llnalys.l.s. We have e:-:tended our PKC s~ 1Jdies ~o include belli9.n; pi:;ernal!gnant:, and l!llllJ.gnan·t tissue biopsies a.s · ' 11 as hUJ>l le to d ::-ec':. th expressior. of PKC ~ : : !Uih transcript:s ln t~mo:: cell line~ fro~ ~clor., brain , bteast, and· hemapoiecic: cells·. ~!.,. have a.l.:so analy't.~d ~ h., t unctior.a:l cole o .! the »KC i~ctypes, and a::-:ci·: it :r o-t th ' l\i;rnan PKC ~ p!'omoi;e•. Transfec~ie Qt.,,::: . fn hum4n melanoma ce:~s cQMPared to human 'elanocyces. These. daca su99e't an alcera~ion in the e xpression ot ~n~ PKC 11 11 1.socype in ~ne t~1.t,.::ifo:cma·t.ion of .~.::r~!: priMr'y melsnocyles , w.?\ i cil ~:i:>y ~er.,e as a th.erapeutic ~arget.