Identification of Thieno[3,2-b]pyrroles as Allosteric Inhibitors of Hepatitis C Virus NS5B Polymerase.

Abstract Thieno[3,2- b ]pyrroles are a novel class of allosteric inhibitors of HCV NS5B RNA-dependent RNA polymerase which show potent affinity for the NS5B enzyme. Introduction of a polar substituent in the position N1 led to a compound that efficiently blocks subgenomic HCV RNA replication in HUH-7 cells with an EC 50 of 2.9 μM.