RP-HPLC Method Development and Validation of Abacavir Sulphate in Bulk and Tablet Dosage Form

RP-HPLC method was developed for the estimation of abacavir sulphate in bulk and pharmaceutical dosage form (tablets) by using INERTSIL ODS 3V column, C18 (250x4.6 ID) mobile phase consisting of a mixture of 10mM phosphate buffer: ACN (60:40 v/v %) P H : 4.0 with detection of 287 nm. The retention time was found to be 2.430min and linearity was observed in the range 60-140µg /ml. Still now there were a number of analytical methods were developed for the estimation of abacavir in pharmaceutical dosage form and also in biological samples like spectroscopic methods, chromatographic methods, etc. But the present method was met the validation parameters according ICH guidelines like accuracy, precision, linearity, range, robustness, ruggedness, limit of detection and limit of quantitation, etc. with a short around time. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2.