螢光測定海による歯肉溝浸出液および血清中の Cathepsin D 活性について

The cathepsin D activities in gingival fluids and sera from clinical healthy subjects were measured by both Anson's (1938) and Schwabe's method (1973). Comparative study of both methods was performed to find out better way for measurement of cathepsin D activity.The following results were obtained;1) By Anson's method, cathepsin D activities could be detected with only 3 samples of 5 gingival fluids, however, all 5 samples were measured sufficiently by Schwabe's method.The activities could be measured with all sera by either methods.2) Cathepsin D activity measured by Schwabe's method showed 5 and 3.3 times higher than those by Anson's method with average value in gingival fluids and sera, respectively.3) Substrate concentration and incubation time applied for Schwabe's method were found enough to be 1/16 and 1/5 respectively compare with those for Anson's method.