Molecular cloning, testicular postnatal expression, and oocyte-activating potential of porcine phospholipase Cζ

The molecular mechanism by which sperm triggers Ca 2+ oscillation, oocyte activation, and early embryonic development has not been clarified. Recently, oocyte activation has been shown to be induced by sperm-specific phospholipase Cζ (PLCζ). The ability of PLCζ to induce oocyte activation is highly conserved across vertebrates. In the present study, porcine PLCζ cDNA was identified and the nucleotide sequence was determined. The expression pattern of porcine PLCζ mRNA during the period of postnatal testicular development was shown to be similar to that of mouse PLCζ. PLCζ mRNA expression in the pig and mouse was detected only in the testes when the elongated spermatids had differentiated, and was detected from day 96 after birth in the pig. Histological examination of porcine testis during the period of postnatal development revealed the presence of spermatozoa from day 110 after birth. These findings suggest that the synthesis of PLCζ mRNA starts when spermiogenesis is initiated. Microinjection of porcine PLCζ complementary RNA into porcine oocytes demonstrated that porcine PLCζ has the ability to trigger repetitive Ca 2+ transients in porcine oocytes similar to that observed during fertilization. It was also found that porcine PLCζ cRNA has the potential to induce oocyte activation and initiate embryonic development up to the blastocyst stage.