Anti-actin antibodies Chemical modification allows the selective production of antibodies to the N-terminal region

Abstract The specific properties of sera elicited by various native, unfolded or chemically modified actins were compared to provide a means of obtaining high titres of antibodies directed against the N-terminal (1–39) or the central regions of the actin sequence. The antigenic structure of the N-terminal region of actin was analyzed. It has at least 2 discrete epitopes, one of which appears to be species-specific and is composed of the hydrophilic N-terminal heptapeptide sequence.