Experimental study of placental amniotic membrane wrapped acellular nerve bridge repair of peripheral nerve defects

Objectives: To study muscle action potentials, sciatic nerve conduction velocity, and explore placenta amni-otic membrane wrapped acellular allogeneic nerve graft. Methods: Animals grouping: groups A and B having 6 dogs each, and group C with only 3 animals. Group A is the allogeneic nerve allograft; Group B: amniotic membrane wrapped acellular nerve transplantation; Group C: allogeneic nerve donor. Groups A and B were anesthetized for bridging nerve allograft. Electrophys-iology, HE and immunohistochemistry were used to observe number of axons; and myelin layers in trans-planted nerves cross-section. Recorded soleus action potentials and bilateral sciatic nerve conduction veloc-ity. Results: Group A showed poor nerve regeneration. Group B showed more visible schwann cells prolifera-tion and large number of regenerative fibers P 0.05. Conclusion: the study demonstrated Improvement in nerve morphology and graft q