Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Sugar Beet Leaf Extracts in Response to Exogenous Application of Resistance Inducers to Manage Sugar Beet Powdery Mildew
2018
The effect of foliar application of some chemical resistance inducers i.e., chitosan, ascorbic acid, salicylic acid and tap water as a control were tested to evaluate their efficacy to induce systemic resistance against Erysiphe betae, the causal agent of sugar beet powdery mildew (Herkl cv.) versus the traditional fungicide Opera under greenhouse and field conditions at Gemmeiza Agric. Res. Sta., Agric. Res. Cent. (ARC), Egypt during two successive winter seasons (2014/2015 and 2015/2016). Under greenhouse conditions, results revealed that spraying plants with all the tested IRCs reduced the disease severity of powdery mildew. The fungicide Opera was the most effective treatment in reducing disease severity and increasing the efficacy followed by chitosan and salicylic acid. Also, all treatments significantly increased total phenols and orthodihydric (OD) phenol content of the leaves compared to the control at 15, 30 and 45 days after inoculation. The GC-MS analysis of methanol extracts of 60 days old leaves of sugar beet plants under the greenhouse conditions, which have been treated with chitosan, ascorbic acid, salicylic acid as inducers to the systemic resistance of sugar beet against Erysiphe betae recorded the highest concentration (peak area %) for all bioactive phytochemical compounds such as flavonoids, alcohols, aldehydes, aromatic compounds, fatty acid methyl esters, terpenoids, phenolics and steroids that can be postulated for antimicrobial activity compared to control (tap water). Under field conditions, results indicated that the fungicide Opera was the most effective treatment for controlling powdery mildew followed by ascorbic acid. Meanwhile, all treatments showed lower effect than the fungicide Opera in this regard but higher than control. All treatments increased root yield per feddan compared to the control. The fungicide Opera and salicylic acid gave the highest root yield per feddan followed by ascorbic acid then chitosan. Moreover, salicylic acid and the fungicide Opera gave the highest percentages of total soluble solids (T.S.S.) and sucrose percentages, whereas salicylic acid, ascorbic acid and fungicide Opera gave the highest percentages of purity in sugar beet roots.
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