Identification of serum metabolites associated with the risk of metritis in transition dairy cows

In this study, we aimed to identify metabolite signatures that characterize metritis prior to, during, and after the disease incidence. Blood samples were collected from 100 Holstein cows at 5 time points before and after parturition. Six cows that developed metritis and 20 controls were selected for metabolomics analysis in a nested case-control study. Twenty nine serum metabolites were quantified using gas chromatography - mass spectroscopy. Results showed that similar panels of metabolites differentiated pre-metritic and CON cows at -8 and -4 wks prepartum. The top most important metabolites that differentiated the two groups of cows at -8 wks prepartum were oxalate, ornithine, pyroglutamic acid, D-mannose, glutamic acid and at -4 wks prepartum were ornithine, pyroglutamic acid, D-mannose, glutamic acid, phosphoric acid, suggesting their potential use as risk biomarkers for metritis. Area under the curve with values of 1.0 and 0.969 at -8 and -4 wks, respectively, indicated that those panels of metabol...