Changes in Gene Expression Associated with Stable Drug and Radiation Resistance in Small Cell Lung Cancer Cells are Similar to those Caused by a Single X-Ray Dose

Abstract Henness, S., Davey, M. W., Harvie, R. M., Banyer, J., Wasinger, V., Corthals, G. and Davey, R. A. Changes in Gene Expression Associated with Stable Drug and Radiation Resistance in Small Cell Lung Cancer Cells are Similar to those Caused by a Single X-Ray Dose. Radiat. Res. 161, 495–503 (2004). Small cell lung cancer (SCLC) initially responds well to chemotherapy and fractionated radiotherapy, but resistance to these treatments eventually develops in the vast majority of cases. To understand how resistance develops in the H69 SCLC cell line, we compared the changes in gene expression associated with 37.5 Gy fractionated X-ray treatment that produced the stable radiation- and drug-resistant H69/R38 cell subline to the changes associated with a single 4- or 8-Gy X-ray treatment. Gene expression was determined by suppression subtractive hybridization combined with Northern blot analysis and two-dimensional (2D) protein electrophoresis. Stable radiation and drug resistance was associated with coordin...