Atrial Natriuretic Peptide

Diuretic and natriuretic extracts, which have been characterized as peptide in nature, have been obtained by homogenization of mammalian heart atria with an aqueous solution of a lower carboxylic acid and may be prepared synthetically. After precipitation of impurities by pH adjustment, the extract may be further purified chromatographically. Extracts injected into test rats resulted in 30-40 fold increases in sodium and chloride excretions within 5-10 minutes of injection. Urine volume rose 10-15 fold and potassium excretion doubled. The response was complete in 20 minutes and no similar changes in renal function were observed following injection of a similarly obtained ventricular extract.