[Expression, localization and interrelationship of P27kip1 and cyclin D3 in non-Hodgkin's lymphoma].

ObjectiveTo investigate the expression, localization and interrelationship of P27 kip1 and cyclin D3 in non-Hodgkin’s lymphoma (NHL). MethodsThe expressions of P27 kip1 , cyclin D3 and index Ki-67 was detected in 100 NHL and 20 reactive lymph nodes by immunohistochemical technique. The expression and localization of P27 kip1 and cyclin D3 in 3 NHL cell lines were detected by Western blot, double immunolabelling and laser scanning confocal microscopy,respectively. ResultsIn general the expression of P27 kip1 in NHL was lower than in control group, and was negatively related to the tumor aggressiveness and proliferating activity; the expression of cyclin D3 in NHL was higher than in control group, and was positively related to the tumor aggressiveness and proliferating activity. There was a negative correlation between P27 kip1 and cyclin D3. Nevertheless, anomalous high P27 kip1 expression was found in a few NHL tissues with high expression of cyclin D3 and Ki-67. Overexpression and colocalization of P27 kip1 and cyclin D3 was found in Raji cell line. ConclusionsUnder expression of P27 kip1 and overexpression of cyclin D3 may play a role in the occurrence and development of NHL. Anomalous high P27 kip1 expression and its interaction with cyclin D3 may be another mechanism for tumorogenesis of NHL.