Analysis of Eggplant Linkage Map Using SRAP Molecular Markers

Molecular markers were valuable tools,widely used in genomic DNA analysis.Several molecular marker techniques had been in the development of DNA markers for map construction,which were useful in breeding,taxonomy,evolution and gene cloning.Based on the genetic analysis of SRAP marker for eggplant,the genetic linkage map was constructed with Mapmanager software by SRAP markers.DNA samples extracted from a F2 population of 255-4-4(S.melongena L.subsp.ovigerum Salis) ×Bai-3(S.melongena L.subsp.melongena).33 of the 88 pairs of primers tested showed polymorphism and stability,ratio of polymorphism band was 37.5 %,40 polymorphic loci were obtained from 33 pairs of primers and average was 1.21,24 of 40 loci were integrated into 4 linkage groups,which covered genome 381.3cM.The average distance between markers was 19.1 cM.