An Interlocked DNA Cascade System for Universal Probe-based Melting Curve Analysis

Single-base mutations are the most common type of mutation in human diseases. The melting curve analysis method is current-ly one of the most commonly used methods for detecting single base mutations. However, the existing melting curve analysis cannot possess universality and robust detection performance simultaneously. Herein, we invented an interlocked DNA cascade system based universal melting curve analysis (ICU-MCA). The strategy is based on the probe dissolution curve method by designing a bridge strand to achieve a good distinction between mutant-type DNA and wild-type DNA. What's more, this method can complete multiplexed detection only by changing the bridge sequence, replacing the specific and expensive probe in the traditional probe based melting curve analysis. We performed 6-plex detection on 6 single-base point mutations in BRAC1 /2 genes on synthetic single stranded DNA and verified the compatibility of ICU-MCA and PCR and detected BRCA1/c.2082C>T and BRCA2/c.7397T>C mutations in the peripheral blood DNA of ovarian cancer patients. Overall, ICU-MCA is one of the best methods in the field of melting curve analysis of detecting single detection mutations.