Advances in the Study of non-classical Inclusion Bodies

Expression of heterologous genes in prokaryotic cell is a fast,simple and cheap way to produce large amount of target proteins,especially recombinant protein drugs.However,overexpression of recombinant protein often leads to the formation of aggregates called inclusion bodies(IBs).In the past,IBs were recognized as deposits of misfolded and inactive proteins,and denaturation/renaturantion steps is necessary for isolation of biologically active protein.Until recently,IBs have been described as biological activity,which can be extracted from non-denaturing conditions,named non-classical Inclusion bodies(ncIBs).This review which focuses on the definition,mechanism and extraction of ncIBs is expected to provide valuable references for the research and exploit of recombination protein productions.