α-Chymotrypsin Immobilized on Chitin. Relationships Between the Enzyme Kinetic Constant and Support Structure
1994
α-Chymotrypsin was immobilized on chitin from squills, lobsters and prawns by means of glutaraldehyde. Hydrolase and peptide synthetase activities were determined in aqueous and homogeneous aqueous-organic media, respectively.The results show α-chymotrypsin immobilized on chitin from prawn to be the most active immobilized derivative based on its synthetase activity (90% yield of Bz-Tyr-Leu-NH2 in carbonate buffer, pH 9 containing 70% 1,4- butanediol).The relationship between the kinetic constant of hydrolysis and chitin structure was also studied. α-Chymotrypsin immobilized on prawn chitin was found to be the best derivative in kinetic terms.The stability of the three derivatives was studied at 37d`C.
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