α-Chymotrypsin Immobilized on Chitin. Relationships Between the Enzyme Kinetic Constant and Support Structure

1994 
α-Chymotrypsin was immobilized on chitin from squills, lobsters and prawns by means of glutaraldehyde. Hydrolase and peptide synthetase activities were determined in aqueous and homogeneous aqueous-organic media, respectively.The results show α-chymotrypsin immobilized on chitin from prawn to be the most active immobilized derivative based on its synthetase activity (90% yield of Bz-Tyr-Leu-NH2 in carbonate buffer, pH 9 containing 70% 1,4- butanediol).The relationship between the kinetic constant of hydrolysis and chitin structure was also studied. α-Chymotrypsin immobilized on prawn chitin was found to be the best derivative in kinetic terms.The stability of the three derivatives was studied at 37d`C.
    • Correction
    • Source
    • Cite
    • Save
    • Machine Reading By IdeaReader
    18
    References
    6
    Citations
    NaN
    KQI
    []