HPLC-TOFMS in rapid separation and identification of chemical components in Oldenlandia diffusa and its injection preparations.

Objective To rapidly separate and identify the chemical components in traditional Chinese herbal medicine of Oldenlandia diffusa and its injection preparations by high performance liquid chromatography-time of flight mass spectrometry(HPLC-TOFMS).Methods An Agilent Zorbax XDB-Cl8 column(250 mm×4.6 mm,5 μm) was used for separation and identification of chemical components in Oldenlandia diffusa,with a mobile phase of 0.3% acetic acid(A) and methanol(B) in gradient elution,0-30 min,30%-90%B.The flow rate was set at 1.0 ml/min and the injection volume was 10 μl.The time-of-flight mass spectrometer was equipped with an EIS ion source.The scanning mass range was between m/z 100-1 000.Results The traditional Chinese medicine of Oldenlandia diffusa and its injection preparation were on-line separated and characterized by HPLC-TOFMS,and 11 chemical compounds were identified in Oldenlandia diffusa,6 compounds in market injection preparation,and 2 compounds in the injection prepared by ourselves.Conclusion Chromatographic demonstration of chemical compounds in Oldenlandia diffusa in one run provides a foundation for the further studying the metabolism and mechanism of Oldenlandia diffusa and its injection preparations.