ANTI-INFLAMMATORY PROPERTIES OF PAVETTA CRASSICAULIS BREMEK. LEAF AND FLOWER CRUDE EXTRACTS AND ITS PURE COMPOUNDS COLLECTED FROM WESTERN GHATS, KARNATAKA, INDIA

Objective: Pavetta crassicaulis Bremek. (F: Rubiaceae) plant extracts were subjected to anti-inflammatory experiment by using carrageenin-induced rat hind paw edema method.Methods: Groups of 5 rats of both sexes (pregnant females excluded) were given a dose of the extract. After 1 h, 0.1 ml, 1% carrageenan suspension in 0.9% NaCl solution was injected into the subplantar tissue of the right hind paw. Paw volume was measured plethysmometrically at 0 h and 3 h after carrageenin injection.Results: Anti-inflammatory experiments revealed that, the leaf and flower ethanolic extract of the P. crassicaulis Bremek. plant shown excellent anti-inflammatory and its extracted pure compounds, 2-tert-butyl-4,6-bis(3,5-di-tert-butyl-4-hydroxybenzyl)phenol and 4H-pyran-4-one,2,3-dihydro3,5- dihydroxy-6-methyl- is a pure compound isolated from the Pavetta crassicaulis flower extract. showed excellent anti-inflammatory activity compared with the standard.Conclusion: P. crassicaulis Bremek. leaf and flower could be exploited as a valuable source of anti-inflammatory agent for the pharmaceutical industry.