Isolation and Identification of Porcine Reproductive and Respiratory Syndrome Virus

To isolate porcine reproductive and respiratory syndrome virus(PRRSV),the morbid materials were collected from some swine farm of Liaoning province and inoculated on Marc-145 cell after disposal.After blind transfer of culture,two portions showed typical cytopathic effect(CPE).TCID50 of virus was 10-6.5/mL.By the indirect immunofluorescence experiment,the yellow green fluorescence occurred in the cell cytoblastema.The virus appeared typical porcine reproductive and respiratory syndrome virus form: diameter was about 50-70 nm,circle with peplos and peplomer.It was inactivated by chloroform,ultra-violet,pH variation,and heat.The cell cytoblastema which showed CPE were tested by RT-PCR showed positive.The laboratory animals appeared obvious clinical symptom and death after inoculation.The result showed that PRRSV was identified from Liaoning province.