Expression and N-glycan Pattern Analysis for Human 90K Glycoprotein in Drosophila S2 Cells

Cancer that is one of serious diseases for human causes angiogenesis, metastasis, andabnormal cell divisions including genetic change. Thus, lots of researchers have beeninvestigated to inhibit cancer. Human 90K (h90K) protein has very crucial role for inhibition of cancer metastasis. h90K is a glycoprotein having potential 7 N-glycosylation sites that 4 sites are clear but remaining 3 sites are not yet. In this work, we performed expression of recombinant h90K glycoprotein in Drosophila S2 cell system. We constructed stablytransfected S2-h90K cells by antibiotic selection and confirmed h90K expression using Western Blot and in vitro bioactivity analyses. We also identified N-glycan patterns of recombinant h90K glycoprotein using HPLC and MALDI-TOF MS. Therefore, this research is going to development of engineered glycosylation for humanized recombinant 90K in Drosophila S2 cells.