Antimicrobial Olympics: Isolation and Characterization ofAntibiotic-Producing Microorganisms
2001
Students work individually to selectively enrich, isolate, purify, and characterize
microorganisms from the soil belonging to two of the groups (the actinomycetes and the fungi) well
known for their ability to produce compounds belonging to the world of secondary metabolites. The
secondary metabolites encompass any materials that are not part of primary metabolism and we know
many of these products such as pigments, odors, and most importantly antibiotics. The students
evaluate isolates for their ability to produce antibiotics as part of a group project. Zones of
inhibition for the various isolates are measured against a panel of test organisms. This laboratory
is an excellent starting point for discussions of antibiotic discovery and use, microbial ecology,
diversity, and antibiotic resistance. Group discussion and a formal report are used to assess the
project.
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