[Study on anti-tumor chemical constituents from pericarps of Juglans mandshurica].

Objective: To study the anti-tumor chemical components of the pericarps of Juglans mandshurica. Methods: The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin,silica gel,Sephadex LH-20 columns and recrystallization. The structures were elucidated on the basis of physicochemical properties and NMR spectral data analysis. Results: From the pericarps of Juglans mandshurica,twelve compounds were separated and identified as 3-methoxy juglone( 1),3-ethoxy juglone( 2),1,8-dihydroxy anthraquinone( 3),juglone( 4),2α,3α,19α-trihydroxy ursolic acid( 5),1α,3β-dihydroxy-olean-18-ene( 6),methyl gallate( 7),pterocarine( 8),quercetin( 9),kaempferol( 10),daucosterol( 11),and β-sitosterol( 12). Conclusion: Compounds 1 ~ 3 and 6 are isolated from the pericarps of Juglans mandshurica for the first time. Compounds 5 and 7 are isolated from Juglans genus for the first time.