The requirement of NKT cells on the generation of Th2 cells from naive T cells stimulated with monocyte chemoattractant protein-1 (MCP-1)
2002
whereas non-atopic controls (3F, 2M; mean age 29) had negative skin prick tests and RAST tests. Furthermore, peripheral blood cells were cultured with either IL-2 + IL-12 or IL-2 + IL-4 to promote cell differentiation towards either Th 1 or Th2 respectively. Results show that in freshly isolated cells, atopic individuals exhibit increased numbers of CCR3+CD4+ (p<0.01; Mann-Whitney U-test) cells compared to normal subjects. However, analysis of T cell cultures shows no significant differences in chemokine receptor expression between normal and atopic donors. Incubation of T cell lines in the presence of IL-2 and the Th2 cytokine IL-4 variably increased CCR3 and CCR4 positive cell numbers. Moreover, conditioning cells with IL-2 + IL12 decreased the expression of CCR3 but had no influence upon CCR4 expression levels. Surprisingly, the Thl associated chemokine receptors, CCR5 and CXCR3, were generally unaffected by culturing cells with 1L12. Analysis of other chemokine receptors, not normally associated with skewed Thl or Th2 responses, showed novel trends in CCR2 expression. CCR2 seemed to be associated with Th2 responses as, like CCR3, expression was increased after culture with IL-4 (p<0.01; ANOVA and Tukey's post test) and decreased after culture with IL-12 (CD4+ cells p<0.05; CD8+ cells p<0.01). These data suggest a potential role for CCR3 in the regulation of atopic rhinitis, support a role for CCR4 in allergic inflammation and suggests that CCR5 is not necessarily Thl-restricted.
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