Purification method for β-nicotinamide mononucleotide

Disclosed is a purification method for β-nicotinamide mononucleotide, comprising the following steps: a. using membrane concentration equipment to perform microfiltration and then nanofiltration on a pre-treated β-nicotinamide mononucleotide solution and collecting a concentrated crude solution; b. adjusting the pH value of the obtained crude solution to be from 3 to 7, injecting sample and applying a preparation column for reversed-phase high performance liquid chromatography, the stationary phase being octadecylsilane bonded silica, the mobile phase A being a solution having the pH value from 3 to 7 formulated using a hydrochloride solution, the mobile phase B being ethanol, and performing purification by gradient elution to obtain a purified sample solution; c. using the membrane concentration equipment to perform nanofiltration on the purified sample solution, using a vacuum freeze dryer to perform lyophilization, and obtaining purified β-nicotinamide mononucleotide.