tert -Butylthiacalix[4]arene monolayers as a biomimetic model for the oxidation of antioxidants with cytochrome c

The possibility of designing a model of sensor to antioxidants based on thiacalix[4]arene monolayers with immobilized cytochrome c was shown. The molecular surface area S 0 of thiacalix[4]arene in the monolayers and the surface pressure coefficient −dπ/dS (elasticity) reflect changes in the redox state of cytochrome c in the presence of dihydroquercetin and ascorbic acid in the aqueous subphase. The absorption spectra in the visible and UV ranges of solutions of the subphase and transferred thiacalix[4]arene monolayers with immobilized cytochrome c confirm the oxidation of the antioxidants to quinones and formation of the reduced form of cytochrome c.