Efecto del 2,4 Diclorofenoxiacético en la inducción de callos a partir de limbos foliares de Ipomoea batatas (L.) Lam. “camote”

Ipomoea batatas (L.) Lam. “camote”, is a rustic crop, of national importance for its energy value and the presence of anthocyanins andcarotenes. Its agronomic management implies its asexual propagation through cuttings, being plant biotechnology a good alternative formassive multiplication, in vitro conservation and/or crop improvement, for this, plant growth regulators are used. One of them is 2.4-Dichlorophenoxyacetic (2.4-D), which at low concentrations behaves as an inducer auxin in the generation of calluses. Given the need forknowledge in this area, it was proposed as a research objective to determine the effect of 2.4-Dichlorophenoxyacetic acid on the inductionof calluses from leaf blades of I. batatas. In the laboratory, explants were grown from previously disinfected leaf blades of young leaves of I.batatas of the yellow variety, in the MS culture medium, supplemented with 2.4-Dichlorophenoxyacetic acid (2.4-D), at the concentrationsof 0 μM; 9.48 μM; 11.3 μM y 13.56 μM, using a completely randomized design. At 50 days of evaluation, no statistically significant differenceswere found, however, at the 9.48 μM concentration of 2.4-D the presence of 100% calluses was observed. It is concluded that 2.4-Dichlorophenoxyacetic acid at a concentration of 9.48 μM is favorable in the induction of calluses from leaf blades of I. batatas (L.) Lam."sweet potato". Keywords: Somatic embryos, limbus, sweet potato, 2,4 Dichlorophenoxyacetic. *Autor para correspondencia: slopezm@unitru.edu.pe DOI: http://dx.doi.org/10.17268/rebiol.2021.41.01.12