SYNTHESIS AND EVALUATION OF A NOVEL C8 ESTER-BONDED STATIONARY PHASE FOR REVERSED-PHASE HPLC

ABSTRACT A novel ester-bonded packing for reversed-phase HPLC (RP-HPLC) was synthesized by reacting octanoic acid with β-(3,4-epoxycyclohexyl) ethyltrimethoxy silane; then, the intermediate product was coupled onto porous silica. Characterization of the prepared packing was carried out with elemental analysis, solid-state 13C NMR, and Fourier transform infrared (FT-IR) analysis.   The chromatographic properties of the novel ester-bonded phase have been investigated in RP-HPLC with acidic, basic, and neutral analytes as probes. The influence of organic modifier and pH of mobile phase and the hydrolytic stability of the packing were also studied. Results showed that the new stationary phase has excellent chromatographic properties in RP-HPLC and can resist hydrolysis between pH=2.5~7.5.