Comparison of Three Methods for Activity Assay of Recombinant Human Hyaluronidase

Objective To detect the activity of recombinant human hyaluronidase by different methods and determine the optimal methods for activity assay of recombinant human hyaluronidase under different conditions.Methods 3,5- dinitrosalicylic acid(DNS),p-dimethylaminobenzaldehyde(DMAB) and turbidimetry methods were used to measure the activity of hyaluronidase from Pichia pastoris fermentation supernatant and gel filtration products,and the results were analyzed.Results The linear ranges of DNS,DMAB and turbidimetry methods were 50-450 U/mL,50-350 U/ mL and 0-10 U/mL,respectively.The RSDs were 2.71%,11.71%and 0.65%,respectively.Conclusion DNS method is suitable for the activity assay of hyaluronidase from the fermentation supernatant and turbidimetry is suitable for mat from gel filtration products.