cDNA of glutathione-S-transferase (GST) gene in ostrinia furnacalis and method for constructing RNAi interference engineering bacteria of GST gene in ostrinia furnacalis

The invention relates to cDNA of a glutathione-S-transferase (GST) gene and a method for constructing RNAi interference engineering bacteria of the GST gene, in particular to cDNA of a glutathione-S-transferase (GST) gene in ostrinia furnacalis and a method for constructing RNAi interference engineering bacteria of the GST gene in the ostrinia furnacalis. The nucleotide sequence and amino acid sequence of the GST gene in the ostrinia furnacalis respectively refer to SEQ ID NO.1 and SEQ ID NO.2. The method comprises the following steps: 1. extracting the total DNA of the ostrinia furnacalis, synthesizing the first cDNA chain, designing primers and carrying out PCR (polymerase chain reaction) amplification to obtain Of-GST-RNAi320; 2. carrying out double digestion on Of-GST-RNAi320 and L4440, recovering the needed target fragments and connecting the target fragments and constructing L4440-Of-GST; and 3. transforming L4440-Of-GST to escherichia coli HT115 (DE3), thus completing the construction. The method is simple to operate, is low in cost and has obvious effect on inhibiting growth and development of the ostrinia furnacalis.