Heparin releasable liver-type lipases of the rat : application of monoclonal antibodies
1987
textabstractFrom the introduction, it will be clear that liver lipase
is involved in the metabolism of lipoproteins. The exact
function(s) of the enzyme in lipoprotein metabolism, however,
still remains to be elucidated which of course is necessary
for the understanding of a possible relationship between liver
lipase activity and regression of atherosclerosis (13). Basic
research on the enzyme is needed to reveal the properties of
the protein itself. In this study, research is carried out on
the molecular structure, the localizaton and the function of
the lipase from the liver and the liver-type lipases that are
present in the adrenal gland and in the ovary of the rat.
Earlier, antibodies against the liver enzyme had been developed.
Such antibodies must be monospecific for the liver
enzyme, well characterized and be usable in sufficient
amounts. Because of these considerations and others (see
below), the isolation of a monoclonal antibody against the rat
liver enzyme has been undertaken.
As compared with the conventional polyclonal antisera, a
monoclonal antibody may have certain advantages. In theory, a
hybridoma cell line can be a long lasting source of a wellcharacterized
antibody. Inhibition of lipase activity by a
monoclonal antibody might reveal how the enzyme is capable in
hydrolyzing different substrates. When using a polyclonal
antiserum for enzyme inhibition, all activity is completely
blocked, most probably because of the interaction of several
antibodies with the lipase. A monoclonal antibody may be useful in localization studies as the problem of cross-reactivity
is often less compared with a polyclonal antibody. For
the production and isolation of monoclonal antibodies, immunological
assays must be developed and adapted for the reliable,
efficient and quick detection of anti-liver lipase antibodies
in hundreds of hybridoma supernatants
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