Protacanthamoeba bohemica sp. n., Isolated from the Liver of Tench, Tinca tinca (Linnaeus, 1758)

A new species of amphizoic amoeba, Protacanthamoeba bohemica sp. n., isolated from the liver of Tinca tinca (Cypriniformes: Cyprinidae) is described. Trophozoites typical of the genus Protacanthamoeba Page, 1981 differ distinctly from those of the type species of the genus in having constantly more numerous and much longer acanthopodia. No relevant ultrastructural distinctions were observed. SSU rRNA gene sequence acquired for P. bohemica (the first within the genus) have been deposited in the GenBank database under accession number AY960120. A phylogenetic analysis based on SSU rRNA gene sequences assigned P. bohemica to the Acanthamoeba and Balamuthia clades.