Genotype of Candida species associated with different conditions of vulvovaginal candidosis

Summary It is unknown whether strains isolated from recurrent vulvovaginal candidosis (RVVC), vulvovaginal candidosis (VVC) and asymptomatic carriers are varying or not in genotyping. Candida isolates were genotyped to determine whether specific types were associated with RVVC and VVC. A total of 97 Candida isolates from RVVC patients (n = 43), from VVC patients (n = 47) and asymptomatic carriers (n = 7) were identified by germ tube test, chlamydospore test, CHROMagar Candida and API20 system and were genotyped by polymerase chain reaction (PCR) fingerprinting employing random amplification of polymorphic DNA (RAPD) assay with nine random primers screened. The RAPD identified distinct genotypes that were shared among isolates from most different individuals. Most of the 85 C. albicans strains failed to corroborate any obvious differences in the genotypic variability in the VVC, RVVC patient groups and carriers by clustering analysis. Only a few individuals were found to harbor a distinct genotype of C. albicans isolates associated with a specific disease condition. Zusammenfassung Candida-Isolate wurden genotypisiert, um zu prufen, obspezifiche Genotypen mit rekurrierender Vulvovaginalcandidose (RVVC), Vulvovaginalcandidose (VVC) und asymptomatischen keimtragerinnen assoziiert werden konnen. Insgesamt 97 Candida-Isolate von RVVC (n = 43), von VVC (n = 47) und asymptomatischen Keimtraagerinnen (n = 7) wurden mittels Keimschlauchtest, Chlamydosporen-Nachweis, auf CHROMagar Candida und mittels API20 identifiziert und mittels RAPD-PCR mit neun Primern genotypisiert. In Clusteranalysen konnten keine bei distinkten Krankheitszustanden vorherrschenden Genotypen festgestellet werden. Nue bei wenigen Patientinnen schien ein bestimmten Krankheitsbild assoziiert zu sein.