Formation of Active Oxygens by Linoleic Acid Peroxidation

The formation of superoxide anion ()and hydrogen peroxide () during linoleic acid peroxidation were investigated in linoleic acid-aqueous system at . Superoxide anion was rapidly generated in the early stage of peroxidation, marked to 0.375 (absorbance at 560mm) in the 12mM linoleic acid (POV below 80millieq./kg) incubated for 1 day and then decreased with time-elapsed. Hydrogen peroxide was also rapidly generated in the early stage of peroxidation regardless of linoleic acid concentration. And, superoxide dismutase(SOD) and catalase greatly inhibited the formation of superoxide anion and hydrogen peroxide, respectively.