Optimization of antifungal activity and chemical study of Terminalia mantaly extracts

Context: We are witnessing an increasing number of mycosis due to a weakened immune system, resistance and the limited number of antifungal agents. Hence the need to develop other antifungal agents with pronounced activity. Methodology: The soxhlet extract obtained from hydroalcoolic extract of Terminalia mantaly bark was chromatographed on Sephadex gel G25. The extracts obtained and a ketoconazole, were tested on the in vitro growth of fungal isolates. A chemical study (phytochemical screening, TLC and HPLC) was carried out. Results: Of the 19 extracts obtained, the F8, F9, F10 and P extracts showed the best activities (MFC = 24.37 µg/mL and 12.18 µg/mL respectively on A. fumigatus and C. albicans, C. neoformans). The chemical study revealed the phenols and quinones that could be responsible for this activity. Conclusion: This extraction resultd in more pronounced antifungal activity which would be due to phenols and/or quinones.