A Novel Oligosaccharide on Bovine Peripheral Myelin Glycoprotein P0

Sulfated oligosaccharides have found in glycoproteins in myelin in the PNS, such as P0 and PASII/ PMP22. These glycoproteins are involved in the interaction of cell membranes and play important roles in the formation and maintenance of myelin sheaths. These functions are due in part to the oligosaccharides in their molecules. Recently, the structures of the oligosaccharides of bovine peripheral myelin P0 were reported, and some of these carr y the HNK - 1 carbohydrate epitope (SO4 - 3GlcAβ1 - 3Galβ1 - 4GlcNAc). In this paper, we identified a novel multisulfated oligosaccharide, MS2, using one - dimensional 1 H - NMR, mass spectrometry, and chemical and enzymatic analyses. MS2, which contains a HNK - 1 epitope, was from a single N - glycosylation site of P0 glycoprotein. The structure of the novel oligosaccharide was: Manα1 - 3Manα1 - 6((3SO4)GlcAβ1 - 3(6SO4)Gal β1 - 4(6SO4)GlcNAcβ1 - 2Manα1 - 3)Manβ1 - 4GlcNAcβ1 - 4(Fucα1 - 6)GlcNAc. In addition to the 3 - O - sulfate present in the HNK - 1 epitope, the oligosaccharide contained a 6 - O - sulfated N - acetylglucosamine residue and a 6 - O - sulfated galactose residue. This implies that both Gal6 - O - sulfotransferase, which has not been reported in the PNS, and GlcNAc 6 - O - sulfotransferase are active in the PNS tissues.