The Biological Function of miR-375 in Vascular Cells Injury Induced by AGEs-mediated Diabetes

Objective: To investigate the expression and biological function of miR-375 in injured vascular cells. Methods: Cloning technology was used to construct miR-375 expression vector and subsequently mir-375 expression plasmid was transfected into injured vascular cells. Meanwhile, we set for further study: Huvec12 as control, injured vascular cells, vascular cells after injury inhibition and cells with miR-375 overexpression. Cells were harvested 24 hours after treatments. The expression of Mtpn, NFκB, profilin1 and sICAM1 were measured in both mRNA and protein level. F-actin was examined via fluorescence staining. Cell apoptosis was assessed by flow cytometry. Results: In injured vascular cells with miR-375 overexpression, the mRNA and protein expression of targeted gene Mtpn declined. It was also observed the reduction of NFκB activity, downregulation of the diabetic vascular injury marker profilin1, restoration of F-actin expression, a decrease of sICAM1 expression and reduced cell apoptosis. Conclusions: miR-375 may inhibit the injuries of vascular cells caused by AGEs-mediated diabetes and may become a new target for gene therapy to treat vascular complications caused by diabetes.