ОБРАЗОВАНИЕ БИОПЛЕНОК ШТАММАМИ ГОСПИТАЛЬНОЙ ФЛОРЫ, ВЫДЕЛЕННЫМИ ИЗ БИОЛОГИЧЕСКИХ СУБСТРАТОВ ПАЦИЕНТОВ, НА ПОВЕРХНОСТИ МАТЕРИАЛОВ И ИЗДЕЛИЙ МЕДИЦИНСКОГО НАЗНАЧЕНИЯ

Aim. To study the ability of hospital-associated strains isolated from the biological substrates of patients oper- ated on under extracorporeal circulation, to form bio fi lms on the surface of medical materials and products. Materials and methods. The formation of bio fi lms of strains of Staphylococcus aureus , Serratia liquefaciens , Klebsiella pneumoniae , Pseudomonas aeruginosa and Acinetobacter spp . isolated from the biological substrates of patients operated on under extracorporeal circulation, on different surfaces (politetraftorotilen, medical poly- ethylene, Polyoxybutirate-to-valerate, silicone, polyvinyl chloride), was studied by a modi fi ed method for the surface of the medical materials and products. Results. The in fl uence of the material nature, as well as hydrophi- lization of the surface, on the ability of hospital-associated strains, isolated from the biological substrates of pa- tients operated on under extracorporeal circulation, to form bio fi lms is studied. It is shown that that certain strains exhibit an increased tendency to bio fi lm formation on more hydrophobic surfaces, e. g., Acinetobacter spp. At the same time the activity of Staphylococcus aureus on silicon surface (hydrophobic surface) is minimal. Other strains almost equally form bio fi lms on hydrophilic and hydrophobic surfaces e.g. Serratia liquefaciens . It was also shown that the surface hydrophilization of PEG to 50% for all the studied strains leads to dramatic reduc- tion of bio fi lm formation. Conclusion. The tendency to form bio fi lms of a particular hospital-associated strain is individual and depends on the nature of the medical material and physical-chemical characteristics of its surface. Hydrophilization of the surface of the medical material is accompanied by a lowered risk of bio fi lm formation.