A Novel Label-free Fluorescence Assay for Dipeptidyl Peptidase 4 Activity Detection Based on Supramolecular Self-Assembly

The article presents a new label-free fluorescence assay based on supramolecular self-assembly of cucurbit[7]uril and specific peptide Gly-Pro-Phe-Gly for monitoring DPP4 activity in clinical samples. It also displays a good potential application in high-throughput screening of DPP4 inhibitors.