Effects of sodium selenite on oxidative damage of human thyrocyte culture in vitro

Objective To investigate the influence of selenium(Se)on oxidative damage of human thyrocytes.Methods The human thyroid epithelium cells(TEC)from normal para-adenoma tissues of patients with thyroid adenoma were cultured.H2O2(0-800 μmol/L)with different concentrations was added into thyrocytes of monolayer culture with or without Se(10-7 mmol/L).The activity of GSH-Px and content of MDA were determined.Results When the thyrocytes exposed to H2O2 for 24 hours,the GSH-Px activity declined but MDA content increased with a relation of dose-effect response.Adding Se(10-7 mmol/L)into cell culture,the activity of GSH-Px was increased,and the content of MDA was descent.Conclusion Selenium could protect thyroid follicular epithelial cell against oxidative damage caused by H2O2.